Fig 1: Prediction and analysis of the upstream miRNAs of FRGs in LUAD. (A) Prediction of the potential miRNAs of FRGs in LUAD examined by starbase. (B,C) The expression of miRNA-101-3p in LUAD was examined by TCGA/GEO databases. (D) The correlation between FRGs and clinical features in LUAD. (E) The prognosis value of miRNA-101-3p in LUAD. (F) ROC curve analyses and AUC values for miRNA-101-3p in LUAD. (G) Pearson’s correlation analysis determined the correlation between miRNA-101-3p and ATP5MC3, CISD1, and PGD expression in LUAD examined by starbase. (H) Explored the potential lncRNAs of miRNA-101-3p in LUAD by starbase. (I,J) The expression of GESC and PVT1 in LUAD was examined by starbase. (K) Pearson’s correlation analysis determined the correlation between miRNA-101-3p and GSEC in LUAD. *p < 0.05, **p < 0.01, ***p < 0.001.
Fig 2: Construction of lncRNA/miRNA/FRG interaction network. (A) Pearson’s correlation analysis determined the correlation between GSEC and ATP5MC3, CISD1, and PGD in LUAD. (B) The expression of GESC in LUAD is examined by the GEO dataset. (C) The subcellular location of GESC is examined by lncLocator and Annolnc2 databases. (D) The coding ability of GESC is examined by coding potential calculator databases. (E) The pathologic stage of GESC in LUAD is examined by GEPIA databases. (F) ROC curve analyses and AUC values for GSEC in LUAD. (G) The prognosis of GESC in LUAD is examined by GEPIA databases. *p < 0.05, **p < 0.01, ***p < 0.001.
Fig 3: Depletion of CISD1 inhibits LUAD cell proliferation and migration. (A,B) The expression of GSEC, FRGs, and miRNA-101-3p in LUAD cell lines was examined by qRT-PCR assay. (C–E) The expression of PGD, ATP5MC3, and CISD1 in LUAD tissue was examined by IHC assay. (F) The expression of CISD1 in H1975 cells after knockdown of CISD1 was examined by qRT-PCR assay. (G,H) The growth curve and colony formation assays were utilized to detect the silencing of CISD1 on the growth of H1975 cells. (I) The transwell assay was utilized to detect the silencing of CISD1 on the migration of H1975 cells. *p < 0.05, **p < 0.01, ***p < 0.001.
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